9th December 2025
- In section 1, 'Overview of the protocol', in the Table under 'Library preparation', timing for the flow cell priming and loading was increased from 5 min to 10 min. And in 'Compatibility of this protocol', MinION Mk1B and MinION Mk1C along with their links to the IT requirements documents were removed as these documents have moved to legacy.
- In sections 3 and 6 for the device branches, the link for the flow cell check was updated.
- In section 6, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device.
- In In section 6, 'Priming and loading the PromethION Flow Cell', the important note to remove the flow cells out of the fridge was moved to the top of this section.
- In section 7, 'Data acquisition and basecalling', links for the MinION Mk1B and MinION Mk1C user manuals were removed as these documents have moved to legacy. And in 'To start a sequencing run in MinKNOW', point 3, the kit used for sequencing was specified for the kit selection page on MinKNOW.
- In 'Troubleshooting', section 11, the video link for the PromethION Flow Cell loading was included alongside the link for the MinION Flow Cell loading. And Guppy was removed as it is no longer applicable.
12/9/2025
PCS_9200_v114_revE_09Dec2025
English
MinION
9th December 2025
- In section 1, 'Overview of the protocol', in the Table under 'Library preparation', timing for the flow cell priming and loading was increased from 5 min to 10 min. And in 'Compatibility of this protocol', MinION Mk1B and MinION Mk1C along with their links to the IT requirements documents were removed as these documents have moved to legacy.
- In sections 3 and 6 for the device branches, the link for the flow cell check was updated.
- In section 6, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device.
- In In section 6, 'Priming and loading the PromethION Flow Cell', the important note to remove the flow cells out of the fridge was moved to the top of this section.
- In section 7, 'Data acquisition and basecalling', links for the MinION Mk1B and MinION Mk1C user manuals were removed as these documents have moved to legacy. And in 'To start a sequencing run in MinKNOW', point 3, the kit used for sequencing was specified for the kit selection page on MinKNOW.
- In 'Troubleshooting', section 11, the video link for the PromethION Flow Cell loading was included alongside the link for the MinION Flow Cell loading. And Guppy was removed as it is no longer applicable.
12/9/2025
PCS_9200_v114_revE_09Dec2025
English
PromethION
9th December 2025
- In section 1, 'Overview of the protocol', in the Table under 'Library preparation', timing for the flow cell priming and loading was increased from 5 min to 10 min. And in 'Compatibility of this protocol', MinION Mk1B and MinION Mk1C along with their links to the IT requirements documents were removed as these documents have moved to legacy.
- In sections 3 and 6 for the device branches, the link for the flow cell check was updated.
- In section 6, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device.
- In In section 6, 'Priming and loading the PromethION Flow Cell', the important note to remove the flow cells out of the fridge was moved to the top of this section.
- In section 7, 'Data acquisition and basecalling', links for the MinION Mk1B and MinION Mk1C user manuals were removed as these documents have moved to legacy. And in 'To start a sequencing run in MinKNOW', point 3, the kit used for sequencing was specified for the kit selection page on MinKNOW.
- In 'Troubleshooting', section 11, the video link for the PromethION Flow Cell loading was included alongside the link for the MinION Flow Cell loading. And Guppy was removed as it is no longer applicable.
11/25/2025
PCS_9200_v114_revD_11Dec2024
English
GridION
11th Dec 2024
- Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
- Addition of links to MinION Mk1D IT requirements and user manual.
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for EPI2ME in "Downstream analysis".
11/25/2025
PCS_9200_v114_revD_11Dec2024
English
MinION
11th Dec 2024
- Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
- Addition of links to MinION Mk1D IT requirements and user manual.
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for EPI2ME in "Downstream analysis".
11/25/2025
PCS_9200_v114_revD_11Dec2024
English
PromethION
11th Dec 2024
- Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
- Addition of links to MinION Mk1D IT requirements and user manual.
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for EPI2ME in "Downstream analysis".
12/11/2024
PCS_9200_v114_revD_11Dec2024
English
GridION
11th Dec 2024
- Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
- Addition of links to MinION Mk1D IT requirements and user manual.
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for EPI2ME in "Downstream analysis".
12/11/2024
PCS_9200_v114_revD_11Dec2024
English
MinION
11th Dec 2024
- Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
- Addition of links to MinION Mk1D IT requirements and user manual.
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for EPI2ME in "Downstream analysis".
12/11/2024
PCS_9200_v114_revD_11Dec2024
English
PromethION
11th Dec 2024
- Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
- Addition of links to MinION Mk1D IT requirements and user manual.
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for EPI2ME in "Downstream analysis".
4/19/2024
PCS_9200_v114_revC_06Dec2023
English
GridION
19 April 2024
- Removed storage recommendations following Rapid Adapter addition
4/19/2024
PCS_9200_v114_revC_06Dec2023
English
MinION
19 April 2024
- Removed storage recommendations following Rapid Adapter addition
4/19/2024
PCS_9200_v114_revC_06Dec2023
English
PromethION
19 April 2024
- Removed storage recommendations following Rapid Adapter addition
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