02 July 2025
- Updated workflow image
- Added information for SQK-PCB114.24 kit format update from vials to plate.
- Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
7/2/2025
PCB_9201_v114_revF_02Jul2025
English
MinION
02 July 2025
- Updated workflow image
- Added information for SQK-PCB114.24 kit format update from vials to plate.
- Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
7/2/2025
PCB_9201_v114_revF_02Jul2025
English
PromethION
02 July 2025
- Updated workflow image
- Added information for SQK-PCB114.24 kit format update from vials to plate.
- Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
6/6/2025
PCB_9201_v114_revE_11Dec2024
English
GridION
11th December 2024
- Addition of links to MinION Mk1D IT requirements and user manual.
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for EPI2ME in "Downstream analysis".
- Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
6/6/2025
PCB_9201_v114_revE_11Dec2024
English
MinION
11th December 2024
- Addition of links to MinION Mk1D IT requirements and user manual.
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for EPI2ME in "Downstream analysis".
- Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
6/6/2025
PCB_9201_v114_revE_11Dec2024
English
PromethION
11th December 2024
- Addition of links to MinION Mk1D IT requirements and user manual.
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for EPI2ME in "Downstream analysis".
- Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
12/11/2024
PCB_9201_v114_revE_11Dec2024
English
GridION
11th December 2024
- Addition of links to MinION Mk1D IT requirements and user manual.
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for EPI2ME in "Downstream analysis".
- Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
12/11/2024
PCB_9201_v114_revE_11Dec2024
English
MinION
11th December 2024
- Addition of links to MinION Mk1D IT requirements and user manual.
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for EPI2ME in "Downstream analysis".
- Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
12/11/2024
PCB_9201_v114_revE_11Dec2024
English
PromethION
11th December 2024
- Addition of links to MinION Mk1D IT requirements and user manual.
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for EPI2ME in "Downstream analysis".
- Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
4/19/2024
PCB_9201_v114_revD_06Dec2023
English
GridION
19 April 2024
- Updated Library storage condition following "Selecting for full-length transcripts by PCR" to 4C for short-term storage.
- Removed storage recommendations following Rapid Adapter addition
4/19/2024
PCB_9201_v114_revD_06Dec2023
English
MinION
19 April 2024
- Updated Library storage condition following "Selecting for full-length transcripts by PCR" to 4C for short-term storage.
- Removed storage recommendations following Rapid Adapter addition
4/19/2024
PCB_9201_v114_revD_06Dec2023
English
PromethION
19 April 2024
- Updated Library storage condition following "Selecting for full-length transcripts by PCR" to 4C for short-term storage.
- Removed storage recommendations following Rapid Adapter addition