Ligation sequencing amplicons - native barcoding (SQK-LSK109 with EXP-NBD104 and EXP-NBD114)

DateVersion numberLanguageDeviceChanges
Mar 10 2023NBA_9093_v109_revO_12Nov2019EnglishGridION
February 2023 - Updated product phase to 'Legacy'
Mar 10 2023NBA_9093_v109_revO_12Nov2019EnglishMinION
February 2023 - Updated product phase to 'Legacy'
August 10th, 2022NBA_9093_v109_revN_12Nov2019EnglishGridion Download 1st August 2022 - Removed references to FFPE in the End-prep step. - Updated the steps for preparing third party reagents in 'Native barcode ligation' and 'Adapter ligation and clean-up' steps.
August 10th, 2022NBA_9093_v109_revN_12Nov2019EnglishMinion Download 1st August 2022 - Removed references to FFPE in the End-prep step. - Updated the steps for preparing third party reagents in 'Native barcode ligation' and 'Adapter ligation and clean-up' steps.
July 14th, 2022NBA_9093_v109_revM_12Nov2019EnglishGridion Download 14th July 2022 - Removed references to discontinued flow cells and updated compatibilities
July 14th, 2022NBA_9093_v109_revM_12Nov2019EnglishMinion Download 14th July 2022 - Removed references to discontinued flow cells and updated compatibilities
July 7th, 2022NBA_9093_v109_revL_12Nov2019EnglishGridion Download July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
July 7th, 2022NBA_9093_v109_revL_12Nov2019EnglishMinion Download July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
April 14th, 2022NBA_9093_v109_revK_12Nov2019EnglishGridion Download 14th April 2022 Protocol name change
April 14th, 2022NBA_9093_v109_revK_12Nov2019EnglishMinion Download 14th April 2022 Protocol name change
April 14th, 2022NBA_9093_v109_revK_12Nov2019EnglishAny Download 14th April 2022 Protocol name change
October 15th, 2021NBA_9093_v109_revJ_12Nov2019EnglishGridion Download October 2021 - Clarified R10.3 flow cell loading recommendations
October 15th, 2021NBA_9093_v109_revJ_12Nov2019EnglishMinion Download October 2021 - Clarified R10.3 flow cell loading recommendations
September 13th, 2021NBA_9093_v109_revI_12Nov2019EnglishGridion Download 13th Sept 2021 - Added tables outlining kit contents in the Equipment and consumables section
September 13th, 2021NBA_9093_v109_revI_12Nov2019EnglishMinion Download 13th Sept 2021 - Added tables outlining kit contents in the Equipment and consumables section
April 29th, 2021NBA_9093_v109_revH_12Nov2019EnglishGridion Download 29th April 2021 - Updated step: end-of-step in the 'End-prep' section to take the end-prepped DNA into the native barcode ligation step.
April 29th, 2021NBA_9093_v109_revH_12Nov2019EnglishMinion Download 29th April 2021 - Updated step: end-of-step in the 'End-prep' section to take the end-prepped DNA into the native barcode ligation step.
April 21st, 2021NBA_9093_v109_revG_12Nov2019EnglishGridion Download 21st April 2021 Inclusion of the optional Adapter Mix II Expansion (EXP-AMII001) kit for instances when a user is running a subset of barcodes in one experiment and needs extra Adapter Mix II for more reactions: - EXP-AMII001 is included in the "Equipment and consumables" section - Explanation of when to use EXP-AMII001 in the "Adapter ligation and clean-up" step
April 21st, 2021NBA_9093_v109_revG_12Nov2019EnglishMinion Download 21st April 2021 Inclusion of the optional Adapter Mix II Expansion (EXP-AMII001) kit for instances when a user is running a subset of barcodes in one experiment and needs extra Adapter Mix II for more reactions: - EXP-AMII001 is included in the "Equipment and consumables" section - Explanation of when to use EXP-AMII001 in the "Adapter ligation and clean-up" step
January 21st, 2021NBA_9093_v109_revF_12Nov2019EnglishGridion Download 21st Jan NEBNext FFPE DNA Repair Mix removed from and DNA repair and end-prep step. This step has also been renamed to 'End-prep'.
January 21st, 2021NBA_9093_v109_revF_12Nov2019EnglishMinion Download 21st Jan NEBNext FFPE DNA Repair Mix removed from and DNA repair and end-prep step. This step has also been renamed to 'End-prep'.
January 20th, 2021NBA_9093_v109_revE_12Nov2019EnglishGridion Download 9th Dec Compatibilities updated for EXP-WSH004
January 20th, 2021NBA_9093_v109_revE_12Nov2019EnglishMinion Download 9th Dec Compatibilities updated for EXP-WSH004
September 14th, 2020NBA_9093_v109_revD_12Nov2019EnglishGridion Download 14th Sept 2020 Removed references to Flongle from the protocol
September 14th, 2020NBA_9093_v109_revD_12Nov2019EnglishMinion Download 14th Sept 2020 Removed references to Flongle from the protocol
March 4th, 2020NBA_9093_v109_revC_12Nov2019EnglishGridion Download 21st Feb 2020 Added 'Library storage recommendations' snippet before flow cell loading step
March 4th, 2020NBA_9093_v109_revC_12Nov2019EnglishMinion Download 21st Feb 2020 Added 'Library storage recommendations' snippet before flow cell loading step
January 23rd, 2020NBA_9093_v109_revB_12Nov2019EnglishGridion Download 23rd January 2020 Replaced DNA input recommendations for R10 flow cells with R10.3: - starting with 1.5-3 µg (or 150-300 fmol) DNA - loading 25-75 fmols onto the flow cell
January 23rd, 2020NBA_9093_v109_revB_12Nov2019EnglishMinion Download 23rd January 2020 Replaced DNA input recommendations for R10 flow cells with R10.3: - starting with 1.5-3 µg (or 150-300 fmol) DNA - loading 25-75 fmols onto the flow cell
November 12th, 2019NBA_9093_v109_revA_12Nov2019English Download
November 12th, 2019native_bc_amplicons_v4EnglishAny Download
November 12th, 2019native_bc_amplicons_v4EnglishGridion Download
November 12th, 2019native_bc_amplicons_v4EnglishMinion Download
September 26th, 2019native_bc_amplicons_v3EnglishGridion Download
September 26th, 2019native_bc_amplicons_v3EnglishMinion Download
September 4th, 2019native_bc_amplicons_v2EnglishGridion Download
September 4th, 2019native_bc_amplicons_v2EnglishMinion Download
August 28th, 2019native_bc_amplicons_v1EnglishGridion Download
August 28th, 2019native_bc_amplicons_v1EnglishMinion Download