10th December 2025
- In section 1, 'Overview of the protocol', under subsection 'Steps in the sequencing workflow' and in section 10, 'Downstream analysis', users are advised to use the Dorado basecaller for analysis. And in 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'.
- In section 2, 'Equipment and consumables', single-use tube format for the SQK-LSK114 kit was removed as this format is no longer applicable.
- In section 5, 'cDNA repair and end-prep', 'Check your flow cell' and section 7, 'Priming and loading the flow cell', 'Complete a flow cell check', the link for the flow cell check in the MinKNOW protocol was replaced with the link for the flow cell check document.
- In section 7, 'Priming and loading the MinION and GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. The video link for
'Priming and loading your flow cell' was updated and in step 2, for all device branches, the instruction for using the single-use tube format for the LSK114 kit was removed. The addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. And an important note was added to the bottom of this section for MinION stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
- Section 8, 'Data acquisition and basecalling' was formatted to align with current protocols.
12/10/2025
DCS_9187_v114_revK_10Dec2025
English
MinION
10th December 2025
- In section 1, 'Overview of the protocol', under subsection 'Steps in the sequencing workflow' and in section 10, 'Downstream analysis', users are advised to use the Dorado basecaller for analysis. And in 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'.
- In section 2, 'Equipment and consumables', single-use tube format for the SQK-LSK114 kit was removed as this format is no longer applicable.
- In section 5, 'cDNA repair and end-prep', 'Check your flow cell' and section 7, 'Priming and loading the flow cell', 'Complete a flow cell check', the link for the flow cell check in the MinKNOW protocol was replaced with the link for the flow cell check document.
- In section 7, 'Priming and loading the MinION and GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. The video link for
'Priming and loading your flow cell' was updated and in step 2, for all device branches, the instruction for using the single-use tube format for the LSK114 kit was removed. The addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. And an important note was added to the bottom of this section for MinION stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
- Section 8, 'Data acquisition and basecalling' was formatted to align with current protocols.
12/10/2025
DCS_9187_v114_revK_10Dec2025
English
PromethION
10th December 2025
- In section 1, 'Overview of the protocol', under subsection 'Steps in the sequencing workflow' and in section 10, 'Downstream analysis', users are advised to use the Dorado basecaller for analysis. And in 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'.
- In section 2, 'Equipment and consumables', single-use tube format for the SQK-LSK114 kit was removed as this format is no longer applicable.
- In section 5, 'cDNA repair and end-prep', 'Check your flow cell' and section 7, 'Priming and loading the flow cell', 'Complete a flow cell check', the link for the flow cell check in the MinKNOW protocol was replaced with the link for the flow cell check document.
- In section 7, 'Priming and loading the MinION and GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. The video link for
'Priming and loading your flow cell' was updated and in step 2, for all device branches, the instruction for using the single-use tube format for the LSK114 kit was removed. The addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. And an important note was added to the bottom of this section for MinION stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
- Section 8, 'Data acquisition and basecalling' was formatted to align with current protocols.
12/12/2024
DCS_9187_v114_revJ_12Dec2024
English
GridION
12 December 2024
- Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024
DCS_9187_v114_revJ_12Dec2024
English
MinION
12 December 2024
- Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024
DCS_9187_v114_revJ_12Dec2024
English
PromethION
12 December 2024
- Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
7/31/2024
DCS_9187_v114_revI_31Jul2024
English
GridION
31 July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
18th April 2024
- Included information for the current incompatibility with the default set-up for wf-transcriptomes with this method.
7/31/2024
DCS_9187_v114_revI_31Jul2024
English
MinION
31 July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
18th April 2024
- Included information for the current incompatibility with the default set-up for wf-transcriptomes with this method.
7/31/2024
DCS_9187_v114_revI_31Jul2024
English
PromethION
31 July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
18th April 2024
- Included information for the current incompatibility with the default set-up for wf-transcriptomes with this method.
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated
07th March 2024
- Updated AMPure Beads (AXP) thaw and storage to be at room temperature in RNA degradation and second strand synthesis step
- Updated library loading recommendations to 35-50 fmols.
07th March 2024
- Updated AMPure Beads (AXP) thaw and storage to be at room temperature in RNA degradation and second strand synthesis step
- Updated library loading recommendations to 35-50 fmols.
07th March 2024
- Updated AMPure Beads (AXP) thaw and storage to be at room temperature in RNA degradation and second strand synthesis step
- Updated library loading recommendations to 35-50 fmols.
19th September 2023
- Updated flow cell loading instructions to include light shield installation
- Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
19th September 2023
- Updated flow cell loading instructions to include light shield installation
- Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
19th September 2023
- Updated flow cell loading instructions to include light shield installation
- Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
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