Date	Version	Language	Device		Changes
5/9/2025	DCM_9210_V114_revE_09May2025	English	GridION		09th May 2025 - Added information for SQK-RPB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM. - Protocol title update
5/9/2025	DCM_9210_V114_revE_09May2025	English	MinION		09th May 2025 - Added information for SQK-RPB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM. - Protocol title update
12/11/2024	DCM_9210_V114_revC_11Dec2024	English	GridION		11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/2024	DCM_9210_V114_revC_11Dec2024	English	MinION		11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
9/30/2024	DCM_9210_V114_revB_30Sep2024	English	GridION		30th September 2024 - Included biosafety warning for pathogenic organisms and recommendation to use appropriate laboratory equipment to protocol introduction. - Incuded biosafety warnings in the preparation method. - Included recommendations to protocol introduction for sample storage following PCR amplification. - Updated PCR step to include EDTA quench reaction following amplification.
9/30/2024	DCM_9210_V114_revB_30Sep2024	English	MinION		30th September 2024 - Included biosafety warning for pathogenic organisms and recommendation to use appropriate laboratory equipment to protocol introduction. - Incuded biosafety warnings in the preparation method. - Included recommendations to protocol introduction for sample storage following PCR amplification. - Updated PCR step to include EDTA quench reaction following amplification.
9/4/2024	DCM_9210_V114_revA_04Sep2024	English	GridION		04th September 2024 Protocol release
9/4/2024	DCM_9210_V114_revA_04Sep2024	English	MinION		04th September 2024 Protocol release