Workflow: bulk transcriptomics
Sequencing full-length transcripts for isoform-level expression analysis
Accurate quantification and characterisation of isoform expression is important to investigate the mechanisms of diseases. Nanopore sequencing can span full-length transcripts in single reads, enabling comprehensive transcriptome characterisation at the isoform level, which simplifies transcriptome annotation to reveal novel transcripts and isoforms.
This end-to-end workflow provides a flexible method to sequence full-length transcripts for isoform-level expression analysis, including extraction, library preparation, high-output sequencing on the PromethIONTM, and simple data analysis recommendations.