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Uncovering the full-length extracellular transcriptome in human blood plasma using long-read cDNA sequencing


Due to the recent rise in transcriptome sequencing of liquid biopsies, the extracellular transcriptome in human plasma is slowly but steadily sharing its secrets. Most RNA sequencing in human plasma is performed using short-read sequencing technology. Given their intrinsic limitations, the fragmentation status of the plasma RNA has remained elusive. To date, full-length RNA sequencing data of human plasma is not available, and the current thinking is that all extracellular RNA in platelet-free plasma is highly degraded.

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