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Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing


Long-read sequencing (LRS) has become a standard approach for transcriptome analysis in recent years. Bovine alphaherpesvirus 1 (BoHV-1) is an important pathogen of cattle worldwide.

This study reports the profiling of the dynamic lytic transcriptome of BoHV-1 using two long-read sequencing (LRS) techniques, the Oxford Nanopore Technologies MinION, and the LoopSeq synthetic LRS methods, using multiple library preparation protocols.

In this work, we annotated viral mRNAs and non-coding transcripts, and a large number of transcript isoforms, including transcription start and end sites, as well as splice variants of BoHV-1. Our analysis demonstrated an extremely complex pattern of transcriptional overlaps.

Authors: Norbert Moldován, Gábor Torma, Gábor Gulyás, Ákos Hornyák, Zoltán Zádori, Victoria A. Jefferson, Zsolt Csabai, Miklós Boldogkői, Dóra Tombácz, Florencia Meyer, Zsolt Boldogkői

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