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Sequoia: An interactive visual analytics platform for interpretation and feature extraction from nanopore sequencing datasets


Sequoia is a visualization tool that allows biologists to explore characteristics of signals generated by the Oxford Nanopore Technologies (ONT) in detail. From Fast5 files generated by ONT, the tool displays relative similarities between signals using the dynamic time warping and the t-SNE algorithms. Raw signals can be visualized through mouse actions while particular signals of interest can also be exported as a CSV file for further analysis. Sequoia consists of two major components: the command-line back-end that performs necessary computations using Python and the front-end that displays the visualization through a web interface. Two datasets are used to conduct a case study in order to illustrate the usability of the tool.

Authors: Ratanond Koonchanok, Swapna Vidhur Daulatabad, Quoseena Mir, Khairi Reda, Sarath Chandra Janga

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