Main menu

Reference-free reconstruction and error correction of transcriptomes from Nanopore long-read sequencing


Long-read sequencing technologies still produce a considerable error rate, averaging around 10% in the case of ONT MinION. Full-length transcript sequencing in eukaryotes have the added challenge of having to deal with introns and small transcript variations (alternative splicing). This presents challenges not only for error correction but also for sequence alignment to reference genomes. We have thus developed RATTLE, a new method for the reference-free reconstruction of transcriptomes from long-read sequencing from Nanopore. RATTLE clusters long (cDNA and RNA) reads using approximate sequence similarity, performs error correction per cluster, and outputs corrected reads and quantified transcripts.

Download the PDF

入门指南

购买 MinION 启动包 Nanopore 商城 测序服务提供商 全球代理商

纳米孔技术

订阅 Nanopore 更新 资源库及发表刊物 什么是 Nanopore 社区

关于 Oxford Nanopore

新闻 公司历程 可持续发展 领导团队 媒体资源和联系方式 投资者 合作者 在 Oxford Nanopore 工作 职位空缺 商业信息 BSI 27001 accreditationBSI 90001 accreditationBSI mark of trust
Chinese flag