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Assessing variants across the human genome with interaction-free sequencing on PromethION


Protocol overview: interaction-free whole-genome sequencing — 2 pages

Overview

This protocol overview introduces the sequencing and analysis of variants across a whole human genome, using a PromethION to deliver high output without the need for interaction with the sequencer once the sample is loaded.

Ideal for those who are new to Oxford Nanopore sequencing or wish to minimise hands-on time, this end-to-end method generates sequencing data with a read length N50 of ~10 kb.

In this protocol overview, you will:

  • Learn the key steps in the sequencing protocol
  • Discover our sequencing workflow, starting from the recommended extraction method, through to primary analysis with EPI2ME
  • Find out our recommended sequencing kit and devices

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