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NCM 2022: Developing new bioinformatic methods to identify modifications from Nanopore sequencing


Extraction of microbial genomes in complex samples requires in silico methods, as monoculture cultivation is often extremely difficult or even impossible. The methylation of DNA represents a useful feature, as different microbial taxonomies have different methylomes, a difference driven primarily by DNA restriction mechanisms. To ensure all motifs are included a de novo detection approach is preferable, such novel modifications and motifs are also included in modification features. Additionally, detection must be possible on shorter metagenomic contigs to be useful in binning.

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