NCM 2022: Developing new bioinformatic methods to identify modifications from Nanopore sequencing
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Extraction of microbial genomes in complex samples requires in silico methods, as monoculture cultivation is often extremely difficult or even impossible. The methylation of DNA represents a useful feature, as different microbial taxonomies have different methylomes, a difference driven primarily by DNA restriction mechanisms. To ensure all motifs are included a de novo detection approach is preferable, such novel modifications and motifs are also included in modification features. Additionally, detection must be possible on shorter metagenomic contigs to be useful in binning.
