Nanopore sequencing of single individual Oikopleura dioica
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Why Oikopleura dioica?
Biological features:
- Global distribution
- Short life cycle (5 days at 20°C)
- Chordate body plan
- Transparent embryonic development
- Separate sexes
Genetic features:
- Most compact genome identified in any chordate, only 70Mb
- >18000 predicted genes
- Small introns (peak at 47 base pairs, 2.4% >1kb)
Why Nanopore sequencing?
- Easy library preparation
- Sequencing in real time
- Long reads
- Accessible
