Miroculus Miro Canvas device use with nanopore sequencing protocols
)
DEVCOM CBC is developing multiple workflows with MinION nanopore sequencing systems, from Oxford Nanopore Technologies (ONT), for rapid sample identification by a wide range of end-users (i.e., those with minimal laboratory training to those proficient in molecular biology techniques). Sequencing efforts currently provide a large amount of data in a relatively short amount of time; however, decreasing the amount of hands-on time is key in making the technology more practical and accessible, even for proficient users. Miroculus has provided an alternative to this time-consuming, hands-on process with their Miro Canvas device. Miroculus has previously demonstrated success with ONT’s genomic DNA utilizing ligation protocols, SQK-LSK 109, 110, 112, and 114.
To assess the utility of this device in the hands of laboratory trained personnel, we tested its ability to successfully produce libraries ready for sequencing with the ONT rapid sequencing kit, SQK-LSK110, with plans to test additional versions in the future.