Main menu

London Calling 2023: Investigating RNA isoform expression using the IsoMix toolkit


Accurate quantification of genes and their mRNA products is essential to understanding health and disease. In humans, processes such as alternative splicing cause almost all genes to express multiple mRNA products (isoforms), which can have different functions. Long-read RNA sequencing can cover an entire mRNA sequence in a single read and so identify and quantify the isoforms present. Therefore, we have developed the IsoMix toolkit to enable the discovery, quantification, and characterization of RNA isoforms in both bulk and single-cell samples. With these tools we have discovered hundreds of novel isoforms of disease-risk genes and their likely proteomic consequences; tracked cell-type specific expression of isoforms during neuronal development; and quantified differential expression of isoforms between regions of the human brain. The characterisation of RNA isoforms with the IsoMix toolkit will enable a vastly improved understanding of gene isoforms and their biological and pathophysiological functions.

Authors: Michael Clark

入门指南

购买 MinION 启动包 Nanopore 商城 测序服务提供商 全球代理商

纳米孔技术

订阅 Nanopore 更新 资源库及发表刊物 什么是 Nanopore 社区

关于 Oxford Nanopore

新闻 公司历程 可持续发展 领导团队 媒体资源和联系方式 投资者 合作者 在 Oxford Nanopore 工作 职位空缺 商业信息 BSI 27001 accreditationBSI 90001 accreditationBSI mark of trust
Chinese flag