Multiplexed, long-read CaptureSeq identifies full-length transcript isoforms in the human brain

Abstract

The architecture of full-length human transcript isoforms remains largely unknown, particularly for large and highly spliced genes. We therefore combined CaptureSeq target enrichment with sample barcoding and long-read nanopore sequencing to characterise the full-length transcript isoforms of >1,400 genes in human post-mortem tissue. We focused on voltage-gated calcium channel genes, which are promising psychiatric drug targets but are also expressed in the cardiovascular system. Isoforms identified as enriched in the brain vs. the cardiovascular system could be brain-selective drug targets. The information gained from our approach has the potential to improve transcriptomic annotations and may also identify novel therapeutic targets.