A guide to investigating methylation in the human genome
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Overview
Epigenetics is the study of chemical modifications that can alter phenotype without altering nucleotide sequence. The most well-characterised and widely studied modification in mammalian including human genomes is 5mC DNA methylation — the addition of a methyl group to a cytosine nucleotide.
In this getting started guide, discover how to sequence epigenetic modifications directly from native DNA molecules without PCR or additional library preparation steps to reveal how methylation impacts phenotype, including its role in regulating gene expression.
In this getting started guide, you will:
- Find out how direct DNA Oxford Nanopore sequencing enhances the analysis of epigenetic modifications
- Discover the benefits of Oxford Nanopore sequencing
- Identify the appropriate Oxford Nanopore sequencing kit for your experiment
- For any questions, see the FAQs for this technique
- Read real-world case studies of how researchers are utilising Oxford Nanopore sequencing to understand aberrant methylation in gene promoters and its association with disease
