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How to sequence microbial isolates with the NO-MISS workflow


In this masterclass, walk through the end-to-end nanopore-only microbial isolate sequencing solution (NO- MISS) workflow — from extraction through to data analysis — to discover how to thoroughly characterise bacterial, fungal, and yeast genomes.

In this masterclass, you will learn:

  • How the rapid, end-to-end NO-MISS workflow enables whole-genome characterisation of bacterial, fungal, and yeast isolates
  • How to prepare and sequence microbial isolate research samples with the NO-MISS workflow
  • How to analyse microbial isolate sequencing data with the EPI2ME platform
  • Where to find useful resources online

Speaker name

Alex Trotter Development Scientist – Translational Development, Oxford Nanopore Technologies

Biography

As part of Translational Development in Oxford Nanopore Technologies, Alex works on prototyping and developing clinical applications of nanopore technology in the infection space. His background is in infection diagnostics and his PhD was on the application of nanopore sequencing to the rapid identification of prosthetic joint infection through metagenomics. Following that, Alex worked as part of the COVID-19 Genomics UK Consortium (COG-UK) using sequencing to track SARS-CoV2 variants at the Quadram Institute in Norwich, before moving to Oxford Nanopore.

Speaker name

Chris Alder Bioinformatician, Oxford Nanopore Technologies

Biography

Chris is a bioinformatics workflow developer within the Customer workflows team at Oxford Nanopore. He helps develop and maintain the analysis workflows within the EPI2ME platform, with a particular focus on those centred around infectious disease. Before joining Oxford Nanopore in 2023, he worked on respiratory metagenomics at Guy’s and St Thomas’ Hospital, and malaria immunology at The Francis Crick Institute.

Authors: Alex Trotter & Chris Alder

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