Harnessing the power of Oxford Nanopore long-read DNA and RNA sequencing for innovative strategies in analysis of non-model organisms in ecogenomics and disease
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Oxford Nanopore long-read DNA-RNA (lrDNA and lrRNA) sequencing is now available to all small research laboratories in terms of cost, accessibility, and genome analysis. Coupled to a repertoire of software genome assemblers and filterprocessing tools, Oxford Nanopore long read DNA-RNA sequencing can deliver chromosome level assemblies of nonmodel organisms.
This Nanopore disruptive OMICS technology has been used in our laboratory for a series of six projects: 1) Surveillance of 6 enteropathogens and COVID-19 in wastewater; 2) Carbapenem resistance; 3) Lyme disease and Borrelia genomic-plasmid diversity; 4) Assembly of the 570 Mb 30 chromosome spruce budworm genome; 5) Rapid on-site detection of cyanobacterial blooms; 6) The quest for novel antimicrobials using iChip technology to culture and isolate novel microorganisms from wild and untapped geographic regions
