First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (formerly Scedosporium prolificans)

Here we describe the sequencing and assembly of the pathogenic fungus Lomentospora prolificans using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. The resulting assembly is highly contiguous, containing a total of 37,627,092 bp with over 98% of the sequence in just 26 scaffolds. Annotation identified 8,896 protein-coding genes. Pulsed-field gel analysis suggests that this organism contains at least 7 and possibly 11 chromosomes, the two longest of which have sizes corresponding closely to the sizes of the longest scaffolds, at 6.6 and 5.7 Mb.

Data availability:

This genome project has been deposited at NCBI/GenBank as BioProject PRJNA392827, which includes the raw read data, assembly, and annotation. The assembly is available under accession NLAX00000000; the version described in this paper is version NLAX01000000. The assembly and annotation is also available from the authors' ftp site at ftp.ccb.jhu.edu/pub/data/assembly/L_prolificans.