Diverse Rhizosphere Bacterial Communities Associated With Wild Edible Mushroom Astraeus asiaticus -Metagenomics Insights
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The symbioses ofectomycorrhizal mushroom and seed-bearing plants have shown to host a rich diversity of microbiota that interact with their hosts. Here, we collected soil samples using standard protocols from Bandhgaon village, West Singhbhum Jharkhand, India to study diverse rhizospheric soil bacterial communities associated with ectomycorrhizal fungi Astraeus asiaticus compared to bacterial communities in bulk soil by using MinION oxford nanopore sequencing of 16S rRNA amplicons. For controls, adjacent bulk soil samples were taken at 20m distances from fungi. A total of 37,240 raw read were analyzed, out of which only 2,664 reads were valid read with 662 OTUs was found in plant roots and bulk soil has 22,848 raw reads, out of which 1,796 were valid reads and has 495 OTUs generated using MinION oxford nanopore sequencing and EzBioCloud 16S database version PKSSU4.0 a data analysis platform to reveal the abundance of phyla -Proteobacteria, Firmicutes, Acidobacteria, Actinobacteria and Gemmatimonadetes. The present work analyzed ectomycorrhizosphere presents a diverse microbiome, which likely influence the functioning of the plant-fungus interactions.The data is available at the NCBI – BioProject ID PRJNA615789, Sequence Read Archive with accession number: SRX8009931 for EcM rhizosphere and SRX8009932 for bulk soil. This is the first report of the rhizospheric microbiome belonging to a wild edible mushroom Astraeus asiaticus.
