Direct RNA nanopore sequencing reveals rapid RNA modification changes following glucose stimulation of human pancreatic beta-cell lines
Mulroney, Taylor, and Lee et al. used Oxford Nanopore direct RNA sequencing to investigate how glucose levels affect RNA modifications in human pancreatic beta cells, uncovering thousands of glucose-responsive changes. The study reveals that these modifications, especially m⁶A, are enriched in type 2 diabetes–related genes and act independently of gene expression, offering new insights into RNA-based regulation in diabetes.
Sample type: human pancreatic beta cell lines