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DeepNano-blitz: A fast basecaller for MinION nanopore sequencers


Motivation
Oxford Nanopore MinION is a portable DNA sequencer that is marketed as a device that can be deployed anywhere. Current base callers, however, require a powerful GPU to analyze data produced by MinION in real time, which hampers field applications.

Results
We have developed a fast base caller DeepNano-blitz that can analyze stream from up to two MinION runs in real time using a common laptop CPU (i7-7700HQ), with no GPU requirements.

The base caller settings allow trading of accuracy for speed, and the results can be used for real time run monitoring (i.e. sample composition, barcode balance, species identification, etc.) or pre-filtering of results for more detailed analysis (i.e. filtering out human DNA from human–pathogen runs).

Availability
DeepNano-blitz has been developed and tested on Linux and Intel processors and is available under MIT license at https://github.com/fmfi-compbio/deepnano-blitz.

Authors: Vladimír Boža, Peter Perešíni, Broňa Brejová, Tomáš Vinař

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