Barcode identification from long reads for analysing single-cell gene expression (BLAZE)
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Background:
The recent development of long-read single-cell RNA sequencing methods has laid the foundation for a more in-depth analysis of isoforms for single cells 1,2,3.
Limitations:
Most long-read single-cell RNA sequencing methodologies require matched short-read single-cell RNA sequencing for the identification of cell barcodes, particularly those using nanopore sequencing due to its higher error rate.
What do we need?
A method which requires only nanopore long reads and is compatible with existing workflows.
