Adaptive Sampling with Oxford Nanopore

Adaptive Sampling is a software-based enrichment and depletion method that targets regions of interest during sequencing with no additional library preparation or PCR steps. With this PCR-free method, you can create digital panels targeting genes in difficult-to-sequence regions that could previously only be characterised through high-depth, whole-genome sequencing. Examples include regions encompassing low-complexity, GC-rich sequences, large SVs, and repeat expansions.

Adaptive Sampling is incorporated into MinKNOW, the operating software that drives all nanopore sequencing devices. With real-time sequencing, it is possible to upload a digital panel as a BED file that represents a region of interest to be identified as the DNA strand passes through a nanopore. This is achieved through the rapid mapping of the beginning of the sequenced strand to the provided reference file.

Find out more about Adaptive Sampling in the Nanopore Know-How blogs: Adaptive Sampling: redefining targeted sequencing and Guided by data: Adaptive Sampling for targeted sequencing.