Main menu

A portable, nanopore-based genotyping platform for near real-time detection of Puccinia graminis f. sp. tritici lineages and fungicide sensitivity


Wheat stem rust, caused by the fungus Puccinia graminis f. sp. tritici (Pgt), threatens global food security. Using Oxford Nanopore sequencing, researchers rapidly genotyped field samples in under 48 hours, detecting lineage and fungicide resistance. This approach enables full-gene coverage, variant phasing, and is deployable in low-resource settings — supporting faster, more informed disease control strategies against this ‘cereal killer’.

Key points:

  • Savva et al. introduce the Pgt MARPLE diagnostics platform: a portable, real-time genotyping tool designed to close the wheat stem rust surveillance gap

  • 165 Pgt isolates were collected from 25 countries across the world

  • Using Oxford Nanopore sequencing of a targeted panel of 276 genes and a standard laptop, MARPLE delivered lineage typing and fungicide resistance profiling within 48 hours of leaf sample collection

  • The method has already been successfully deployed in Kenya and Ethiopia, placing powerful genomic tools in the hands of local teams and enabling faster, more informed responses to emerging outbreaks

'The advent of the portable MinION sequencer has unlocked new possibilities for point-of-care disease diagnostics'

Savva et al. 2025

Sample type: fungal isolates

Kit: Rapid Barcoding Kit

Authors: Loizos Savva, Anthony Bryan, Dominik Vinopal, Oscar E. Gonzalez-Navarro, Zennah Kosgey, Kimani Cyrus Ndung’u, Jemal Tola Horo, Kitessa Gutu Danu, Messele Molla, Yoseph Alemayehu, David P. Hodson, Diane G.O. Saunders

入门指南

购买 MinION 启动包 Nanopore 商城 测序服务提供商 全球代理商

纳米孔技术

订阅 Nanopore 更新 资源库及发表刊物 什么是 Nanopore 社区

关于 Oxford Nanopore

新闻 公司历程 可持续发展 领导团队 媒体资源和联系方式 投资者 合作者 在 Oxford Nanopore 工作 职位空缺 商业信息 BSI 27001 accreditationBSI 90001 accreditationBSI mark of trust
Chinese flag