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Reduced representation methylation multiplex sequencing (RRMS) from cells using SQK-NBD114.24 (RRMS_9209_v114_revH_02Jul2025)

DateVersionLanguageDeviceChanges
7/8/2025RRMS_9209_v114_revH_02Jul2025EnglishPromethION
02 July 2025 - Updated information for new kit format with increased Short Fragment Buffer (SFB) volume. - Increased the timing for priming and loading the flow cell from 5 min to 10 min in Library preparation Table.
7/2/2025RRMS_9209_v114_revH_02Jul2025EnglishPromethION
02 July 2025 - Updated information for new kit format with increased Short Fragment Buffer (SFB) volume. - Increased the timing for priming and loading the flow cell from 5 min to 10 min in Library preparation Table.
6/13/2025RRMS_9209_v114_revG_09May2025EnglishPromethION
09 May 2025 - Protocol title update
4/29/2025RRMS_9209_v114_revF_29Apr2025EnglishPromethION
29 April 2025 - Updated "DNA fragmentation" and "DNA repair and end-prep" steps with correction to the expected DNA recovery and quantities taken forward into the library preparation
1/7/2025RRMS_9209_v114_revE_07Jan2025EnglishPromethION
07 January 2025 - Updated "DNA repair and end prep" incubation step to 20°C for 5 minutes, 65°C for 5 minutes. (was 20°C for 5 minutes, 60°C for 5 minutes)
1/7/2025RRMS_9209_v114_revE_13Dec2024EnglishPromethION
07 January 2025 - Updated "DNA repair and end prep" incubation step to 20°C for 5 minutes, 65°C for 5 minutes. (was 20°C for 5 minutes, 60°C for 5 minutes)
12/13/2024RRMS_9209_v114_revD_13Dec2024EnglishPromethION
13 December 2024 - Addition of MinION Mk1D device for RRMS in "Overview of the protocol". - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
11/15/2024RRMS_9209_v114_revC_20Nov2024EnglishPromethION
November 2024 - Updated kit content information and image to include new kit format of SQK-NBD114.24 with plated native barcodes.
8/20/2024RRMS_9209_v114_revB_31Jul2024EnglishPromethION
21 August 2024 - Updated downstream analysis section: recommended software versions. - Included demux step in downstream analysis section.
August 20th, 2024RRMS_9209_v114_revA_31Jul2024English- Download Protocol release

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