Ligation sequencing gDNA V14 — whole genome amplification (SQK-LSK114) (WAL_9192_v114_revI_14May2025)

DateVersionLanguageDeviceChanges
5/14/2025WAL_9192_v114_revI_14May2025EnglishGridION
14th May 2025 - In section 3 'Whole genome amplification', step 13, and section 4 'DNA repair and end-prep', step 4, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included.
5/14/2025WAL_9192_v114_revI_14May2025EnglishMinION
14th May 2025 - In section 3 'Whole genome amplification', step 13, and section 4 'DNA repair and end-prep', step 4, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included.
5/14/2025WAL_9192_v114_revI_14May2025EnglishPromethION
14th May 2025 - In section 3 'Whole genome amplification', step 13, and section 4 'DNA repair and end-prep', step 4, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included.
5/1/2025WAL_9192_v114_revH_01May2025EnglishGridION
01 May 2025 - Updated any wording referring to QIAGEN REPLI-g Midi kit, to now refer to the kit used in the method: REPLI-g Single Cell Kit.
5/1/2025WAL_9192_v114_revH_01May2025EnglishMinION
01 May 2025 - Updated any wording referring to QIAGEN REPLI-g Midi kit, to now refer to the kit used in the method: REPLI-g Single Cell Kit.
5/1/2025WAL_9192_v114_revH_01May2025EnglishPromethION
01 May 2025 - Updated any wording referring to QIAGEN REPLI-g Midi kit, to now refer to the kit used in the method: REPLI-g Single Cell Kit.
1/20/2025WAL_9192_v114_revG_20Jan2024EnglishGridION
20 January 2025 - Removed Covaris g-Tube from equipment list as it is not used in the method.
1/20/2025WAL_9192_v114_revG_20Jan2024EnglishMinION
20 January 2025 - Removed Covaris g-Tube from equipment list as it is not used in the method.
1/20/2025WAL_9192_v114_revG_20Jan2024EnglishPromethION
20 January 2025 - Removed Covaris g-Tube from equipment list as it is not used in the method.
12/12/2024WAL_9192_v114_revF_12Dec2024EnglishGridION
12 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024WAL_9192_v114_revF_12Dec2024EnglishMinION
12 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024WAL_9192_v114_revF_12Dec2024EnglishPromethION
12 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
8/21/2024WAL_9192_v114_revE_26Jul2023EnglishGridION
21st August 2024 - Updated protocol introduction to trimmed down format. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method.
8/21/2024WAL_9192_v114_revE_26Jul2023EnglishMinION
21st August 2024 - Updated protocol introduction to trimmed down format. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method.
8/21/2024WAL_9192_v114_revE_26Jul2023EnglishPromethION
21st August 2024 - Updated protocol introduction to trimmed down format. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method.
August 21st, 2024WAL_9192_v114_revD_26Jul2023EnglishPromethion Download 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
August 21st, 2024WAL_9192_v114_revD_26Jul2023EnglishGridion Download 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
August 21st, 2024WAL_9192_v114_revD_26Jul2023EnglishMinion Download 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
September 19th, 2023WAL_9192_v114_revB_26Jul2023EnglishPromethion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated
September 19th, 2023WAL_9192_v114_revB_26Jul2023EnglishGridion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated
September 19th, 2023WAL_9192_v114_revB_26Jul2023EnglishMinion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated
August 1st, 2023WAL_9192_v114_revA_26Jul2023EnglishPromethion Download -
August 1st, 2023WAL_9192_v114_revA_26Jul2023EnglishGridion Download -
August 1st, 2023WAL_9192_v114_revA_26Jul2023EnglishMinion Download -