During our first instalment of the series, an amazing set of speakers shared how they are using single-cell nanopore sequencing in the laboratory. To start, Daniel Garalde provided an overview of Oxford Nanopore’s solution for full-length sequencing of single cell and spatial assays. Ruli Gao presented a method for processing high-throughput single-cell nanopore RNA sequencing data and Ruta Sahasrabudhe shared her and her team's experience of sequencing single-cell cDNA libraries on the PromethION.

During our second installment of the series, the speakers shared how nanopore sequencing can be used to validate plasmids. Members of the Oxford Nanopore team took us through the protocol for plasmid sequencing using Oxford Nanopore Rapid Barcoding Kits and how to approach analysing the data created. When then heard from our guest speaker, Mel Englund, Postdoctoral Researcher in the Boyle Lab at the University of Michigan. Mel discussed using nanopore sequencing for rapid, multiplexed validation of plasmids.

High-throughput library preparation for next-generation sequencing (NGS) requires a lot of repetitive pipetting and therefore is prone to errors. The process needs experience, precision, and accuracy to generate high-quality libraries. During this webinar, Steph Wilbraham from Oxford Nanopore, shared a comprehensive overview of simple and efficient automated library preparation protocols for nanopore sequencing.