Sequencing and analysis of nanopore-only microbial isolates with the NO-MISS workflow
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Whole-genome sequencing of microbial isolates provides valuable information for public health, clinical microbiology research, food safety, and microbial ecology. Nanopore sequencing provides distinct advantages when it comes to detection and characterisation of antimicrobial resistance, virulence genes, plasmids and other mobile genetic elements.
This knowledge exchange will walk through the nanopore-only microbial isolate sequencing solution (NO-MISS): a flexible and rapid approach for whole-genome sequencing of bacterial isolates.
In this knowledge exchange, viewers will learn:
How the rapid, end-to-end NO-MISS workflow enables whole-genome characterisation of bacterial isolates.
How to extract nucleic acids, prepare libraries, and sequence microbial isolate research samples with the NO-MISS workflow.
How to analyse microbial isolate sequencing data with the EPI2ME platform.
Where to find useful resources online.
Meet the speakers
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Anna Maria Niewiadomska, Segment market manger – public health, Oxford Nanopore TechnologiesIn her role as segment market manager, Anna drives the development of new markets in the infectious disease and public health space. She received her Ph.D. in molecular microbiology and immunology from the Johns Hopkins School of Public Health. Prior to joining Oxford Nanopore, Anna worked on multiple projects focusing on host/virus interactions, mathematical modeling of antimicrobial resistance, microbial bioinformatics, and emerging infectious disease outbreaks. She combines a deep knowledge of experimental and in silico methods for generation and analysis of microbial genomic data.
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Alex Trotter, Development scientist – translational development, Oxford Nanopore TechnologiesAs part of Translational Development in Oxford Nanopore Technologies, Alex works on prototyping and developing clinical applications of nanopore technology in the infection space. His background is in infection diagnostics and his PhD was on the application of nanopore sequencing to the rapid identification of prosthetic joint infection through metagenomics. Following that, Alex worked as part of the COVID-19 Genomics UK Consortium (COG-UK) using sequencing to track SARS-CoV2 variants at the Quadram Institute in Norwich, before moving to Oxford Nanopore.
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Chris Alder, Bioinformatician, Oxford Nanopore TechnologiesChris is a bioinformatics workflow developer within the Customer workflows team at Oxford Nanopore. He helps develop and maintain the analysis workflows within the EPI2ME platform, with a particular focus on those centered around infectious disease. Before joining Oxford Nanopore in 2023, he worked on respiratory metagenomics at Guy’s and St Thomas’ Hospital, and malaria immunology at The Francis Crick Institute.
