Whole-genome sequencing of microbial isolates provides valuable information for public health, clinical microbiology research, food safety, and microbial ecology. Nanopore sequencing provides distinct advantages when it comes to detection and characterisation of antimicrobial resistance, virulence genes, plasmids and other mobile genetic elements.

This knowledge exchange will walk through the nanopore-only microbial isolate sequencing solution (NO-MISS): a flexible and rapid approach for whole-genome sequencing of bacterial isolates.

In this knowledge exchange, viewers will learn:

• How the rapid, end-to-end NO-MISS workflow enables whole-genome characterisation of bacterial, fungal, and yeast isolates.

• How to extract nucleic acids, prepare libraries, and sequence microbial isolate research samples with the NO-MISS workflow.

• How to analyse microbial isolate sequencing data with the EPI2ME platform.

• Where to find useful resources online.