How direct RNA nanopore sequencing can enhance your research
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Nanopore sequencing is the only available technology that directly reads native RNA transcripts, making it possible to quantify gene and isoform expression without PCR bias and detect RNA modifications. With long nanopore reads, isoforms can be resolved in full and poly-A tail lengths accurately estimated. In this knowledge exchange, discover how direct RNA nanopore sequencing works and how it can enhance your research.
In this knowledge exchange, viewers will learn:
How the Direct RNA Sequencing Kit enables the preparation and nanopore sequencing of native RNA, including the detection of RNA modifications.
About the direct RNA sequencing workflow, including how to prepare libraries with the Direct RNA Sequencing Kit, which sequencing device to choose to meet experimental goals, and how to analyse data.
What direct RNA nanopore sequencing can bring to research across numerous applications, with a worked example.
Meet the speakers
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Libby Snell, Director, RNA and cDNA Sample Technology (R&D), Oxford NanoporeLibby Snell, PhD, is the Director of RNA and cDNA Sample Technology, overseeing the R&D and applications of Oxford Nanopore Technologies’ RNA-based sequencing. She is an original designer and developer of direct RNA sequencing, as well as novel cDNA sequencing methods. Libby also leads the technical development of Oxford Nanopore’s mRNA QC identity and integrity test methodologies for biopharma cGMP. She has been with the company for over twelve years. She has a broad background in transcriptomics, molecular genetics, comparative genomics, and evolution & developmental biology from her MS, PhD and postdoctoral research (USA and UK) prior to her time at the company.
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Daniel Garalde, Associate Director - Technology Markets, Oxford NanoporeDaniel used nanopores to study single molecule DNA-protein complexes in the nanopore research group at University of California Santa Cruz where he completed a Ph.D in computer engineering. At Oxford Nanopore Daniel drove forward-looking research projects including development of nanopore cDNA and Direct RNA sequencing. Daniel currently works to bring novel Oxford Nanopore solutions including full-length single cell sequencing and ultra-rapid WGS to key markets.
