Optimized DNA Extracting Method for Oxford Nanopore- Long reads Sequencing from Marine samples
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Qatar’s particular geographic location represents a marine barrier for different species that have migrated from the neighboring environments. Due to the extreme temperatures and salinities in the gulf region, the national biodiversity has adapted to survive under extreme conditions. Furthermore, the barriers that isolates the Arabian Gulf has created an environment that is rich with endemic species that are specific to the region. The aims of our project is to identify the potential new marine species at Qatar Marine Zone trough whole genome sequencing using Oxford Nanopore Technology (ONT). DNA extracted from marine samples is known to be particularly challenging to sequence due to unknown reasons. Therefore, we surveyed a wide range of genomic DNA extraction protocols to assess their efficiency in providing high quality DNA that is suitable for long read sequencing.