Novel Method for High Molecular Weight (HMW) DNA Extraction
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Extraction of high molecular weight (HMW) genomic DNA (gDNA) is currently a pain point for many researchers, with existing solutions taking hours, or even days, to complete. Under the Monarch® Nucleic Acid Purification product portfolio, we have developed a rapid and reliable process for extracting intact HMW genomic DNA from mammalian tissues, cultured cells, blood, and bacteria. Utilizing an optimized process that combines gentle lysis with tunable fragment length generation, followed by precipitation of extracted DNA onto the surface of glass beads, the prep proceeds rapidly and utilizes standard laboratory equipment. DNA size ranges from 50-250 kb for the standard protocol and can be adjusted to produce longer DNA up to >1 Mb. Purified DNA is recovered in high yield with excellent purity, including nearly complete removal of RNA. Prep time is approximately 30-90 minutes, depending on sample type. The purified HMW gDNA is suitable for a variety of downstream applications including long-read sequencing and genome mapping.