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NCM 2022: Visualization and analysis of nanopore signal for modification detection and more with Uncalled4


Nanopore sequencing works by measuring ionic current as individual DNA or RNA molecules pass through a pore. Many analyses utilize direct alignments of the electrical signal to a nucleotide reference, including detection of nucleotide modifications, polishing, and adaptive sampling. Building off methods developed in Uncalled v1, we present Uncalled4: a toolkit for nanopore signal alignment, analysis, and visualization.

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