NCM 2022: Native molecule whole genome sequencing of short fragment cfDNA and FFPE DNA
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The Oxford Nanopore Technologies (ONT) sequencing platform is a promising tool in cancer diagnostics because it enables sequencing of DNA molecules of any length. Furthermore, nanopore sequencing can interrogate epigenetic methylation status without any template manipulation, making it valuable for rapid disease classification and sample stratification. Nanopore technology has been largely optimized for long-read sequencing. However, useful templates like cell-free DNA (cfDNA) and DNA derived from formalin-fixed paraffin embedded tissue (FFPE DNA) are highly fragmented.