Metagenomic approach for eDNA sequencing with Oxford Nanopore MinION to detect elusive Alaskan species in a mesocosm
- Published on: June 4 2018
- Source: London Calling 2018
)
We assess the possibility of processing species monitoring surveys through eDNA with Oxford Nanopore MinION and a metagenomic sequencing approach. We use a mesocosom (fish tank where two live individuals of Alaskan chubs (Couesius plumbeus) for this study along with Halibut DNA as an internal control. Detection is possible despite a low sensitivity. Low variance of the detection rates is encouraging for further improvements.
