Comprehensive characterisation of the mouse retina transcriptome using long-read RNA sequencing
Webinar Overview
In this webinar, Rui Chen evaluates Oxford Nanopore RNA sequencing technology for transcriptome profiling at the single-cell level, and for the generation of a comprehensive atlas of full-length transcript isoforms in the mouse retina. Chen’s team profiled approximately 30,000 mouse retina cells using a combination of 1.4 billion long reads with nanopore sequencing and 1.5 billion short reads with legacy sequencing.
What you will learn:
Nanopore long-read scRNA-seq is highly effective for the identification of isoforms
Isoforms exhibit differential expression across cell class and cell subclass
Although rare, over 2,000 cell class specific isoforms were identified by the lab, with potential use going forward as a cell class marker.
