A sensitive sample preparation pipeline for adventitious virus detection using Oxford Nanopore sequencing
Oxford Nanopore sequencing, paired with the new CoNS-seq sample preparation workflow, enabled rapid and sensitive detection of viral contaminants during cell and gene therapy manufacturing. By enriching for viral genomes even at very low levels, this method could accelerate product release and boost safety — helping critically ill patients access therapies sooner in the future.
'Unlike short-read sequencing, Oxford Nanopore sequencing has real-time basecalling and alignment, which can reduce the overall runtime'
Tsinda and Swofford et al. 2025
Sample type: Jurkat T cells spiked with viruses