Main menu

Nanopore direct RNA sequencing detects differential expression between human cell populations


A crucial requirement of RNA sequencing is identifying differential expression to understand cell functions, differentiation and disease. The development of long-read direct RNA (dRNA) sequencing may overcome many limitations of short-read sequencing. Our study investigates the ability of dRNA sequencing to identify differential gene and isoform expression in both synthetic controls and human cells. We show that dRNA sequencing accurately quantifies RNA and identifies differential expression between conditions. We further identified thousands of novel transcripts and splice isoforms. Our results establish the ability of dRNA sequencing to identify biologically relevant differences in gene and isoform expression and perform the key capabilities of expression profiling methodologies.

Authors: Josie Gleeson

Getting started

Buy a MinION starter pack Nanopore store Sequencing service providers Channel partners

Nanopore technology

Subscribe to Nanopore updates Resources and publications What is the Nanopore Community

About Oxford Nanopore

News Company timeline Sustainability Leadership team Media resources & contacts For investors For partners Working at Oxford Nanopore Current vacancies Commercial information BSI 27001 accreditationBSI 90001 accreditationBSI mark of trust
Spanish flag