Single-cell transcriptomics with cDNA prepared using 10X Genomics

DateVersion numberLanguageDeviceChanges
Dec 6 2023SST_v9148_v111_revH_12Jan2022EnglishGridION
December 2023 - Updated product phase to 'Legacy'
Dec 6 2023SST_v9148_v111_revH_12Jan2022EnglishMinION
December 2023 - Updated product phase to 'Legacy'
Dec 6 2023SST_v9148_v111_revH_12Jan2022EnglishPromethION
December 2023 - Updated product phase to 'Legacy'
September 19th, 2023SST_v9148_v111_revF_12Jan2022EnglishPromethion Download 11th July 2023 - Updated the PCR step in "Pre-pull-down PCR" to clarify the touchdown step. - Added clarification of compatibilities with other 10X Genomics kits. - Added an example for the magnetic rack to use in this protocol.
September 19th, 2023SST_v9148_v111_revF_12Jan2022EnglishGridion Download 11th July 2023 - Updated the PCR step in "Pre-pull-down PCR" to clarify the touchdown step. - Added clarification of compatibilities with other 10X Genomics kits. - Added an example for the magnetic rack to use in this protocol.
September 19th, 2023SST_v9148_v111_revF_12Jan2022EnglishMinion Download 11th July 2023 - Updated the PCR step in "Pre-pull-down PCR" to clarify the touchdown step. - Added clarification of compatibilities with other 10X Genomics kits. - Added an example for the magnetic rack to use in this protocol.
February 8th, 2023SST_v9148_v111_revE_12Jan2022EnglishPromethion Download February 2023 - Removed the checklist download to fix formatting issues.
February 8th, 2023SST_v9148_v111_revE_12Jan2022EnglishGridion Download February 2023 - Removed the checklist download to fix formatting issues.
February 8th, 2023SST_v9148_v111_revE_12Jan2022EnglishMinion Download February 2023 - Removed the checklist download to fix formatting issues.
January 6th, 2023SST_v9148_v111_revD_12Jan2022EnglishPromethion Download January 2023 - Changed the PromethION "Priming and loading the flow cell" section to the updated version.
January 6th, 2023SST_v9148_v111_revD_12Jan2022EnglishGridion Download January 2023 - Changed the PromethION "Priming and loading the flow cell" section to the updated version.
January 6th, 2023SST_v9148_v111_revD_12Jan2022EnglishMinion Download January 2023 - Changed the PromethION "Priming and loading the flow cell" section to the updated version.
December 2nd, 2022SST_v9148_v111_revC_12Jan2022EnglishPromethion Download 2nd December 2022 The "Downstream analysis" section has been updated with a description of the wf-single-cell workflow from EPI2ME Labs.
December 2nd, 2022SST_v9148_v111_revC_12Jan2022EnglishGridion Download 2nd December 2022 The "Downstream analysis" section has been updated with a description of the wf-single-cell workflow from EPI2ME Labs.
December 2nd, 2022SST_v9148_v111_revC_12Jan2022EnglishMinion Download 2nd December 2022 The "Downstream analysis" section has been updated with a description of the wf-single-cell workflow from EPI2ME Labs.
August 10th, 2022SST_v9148_v111_revB_12Jan2022EnglishPromethion Download 11th May 2022 In "Post-pulldown PCR", two new steps have been added at the end: to pellet the beads on a magnet, and retain 15 µl of eluate.
August 10th, 2022SST_v9148_v111_revB_12Jan2022EnglishGridion Download 11th May 2022 In "Post-pulldown PCR", two new steps have been added at the end: to pellet the beads on a magnet, and retain 15 µl of eluate.
August 10th, 2022SST_v9148_v111_revB_12Jan2022EnglishMinion Download 11th May 2022 In "Post-pulldown PCR", two new steps have been added at the end: to pellet the beads on a magnet, and retain 15 µl of eluate.
January 12th, 2022SST_v9148_v111_revA_12Jan2022EnglishPromethion Download
January 12th, 2022SST_v9148_v111_revA_12Jan2022EnglishGridion Download
January 12th, 2022SST_v9148_v111_revA_12Jan2022EnglishMinion Download