Rapid sequencing DNA - PCR Barcoding (SQK-RPB004)

DateVersion numberLanguageDeviceChanges
Sep 7 2023RPB_9059_v1_revQ_14Aug2019EnglishGridION
6th September 2023 - Updated product phase to Legacy
Sep 7 2023RPB_9059_v1_revQ_14Aug2019EnglishMinION
6th September 2023 - Updated product phase to Legacy
September 6th, 2023RPB_9059_v1_revP_14Aug2019EnglishGridion Download 9th August 2022 - Added information about the RAP Top-Up kit in the Equipment and Consumables section as well as splitting libraries in the Library Prep section.
September 6th, 2023RPB_9059_v1_revP_14Aug2019EnglishMinion Download 9th August 2022 - Added information about the RAP Top-Up kit in the Equipment and Consumables section as well as splitting libraries in the Library Prep section.
July 4th, 2022RPB_9059_v1_revO_14Aug2019EnglishGridion Download July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
July 4th, 2022RPB_9059_v1_revO_14Aug2019EnglishMinion Download July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
April 14th, 2022RPB_9059_v1_revN_14Aug2019EnglishGridion Download 14th April 2022 Protocol name change
April 14th, 2022RPB_9059_v1_revN_14Aug2019EnglishMinion Download 14th April 2022 Protocol name change
April 14th, 2022RPB_9059_v1_revN_14Aug2019EnglishAny Download 14th April 2022 Protocol name change
September 13th, 2021RPB_9059_v1_revM_14Aug2019EnglishGridion Download 13th Sept 2021 - Added table outlining kit contents in the Equipment and Consumables section.
September 13th, 2021RPB_9059_v1_revM_14Aug2019EnglishMinion Download 13th Sept 2021 - Added table outlining kit contents in the Equipment and Consumables section.
January 20th, 2021RPB_9059_v1_revL_14Aug2019EnglishGridion Download 9th Dec Compatibilities updated for EXP-WSH004
January 20th, 2021RPB_9059_v1_revL_14Aug2019EnglishMinion Download 9th Dec Compatibilities updated for EXP-WSH004
September 27th, 2019RPB_9059_v1_revK_14Aug2019EnglishGridion Download 27th September 2019 Updated and expanded Troubleshooting guide
September 27th, 2019RPB_9059_v1_revK_14Aug2019EnglishMinion Download 27th September 2019 Updated and expanded Troubleshooting guide
August 23rd, 2019RPB_9059_v1_revJ_14Aug2019EnglishGridion Download 23rd August 2019 Troubleshooting guide added to the protocol, detailing the most common issues seen during a nanopore sequencing experiment.
August 23rd, 2019RPB_9059_v1_revJ_14Aug2019EnglishMinion Download 23rd August 2019 Troubleshooting guide added to the protocol, detailing the most common issues seen during a nanopore sequencing experiment.
August 14th, 2019RPB_9059_v1_revI_14Aug2019EnglishGridion Download 14th August 2019 The protocol has been expanded to include instructions for library prep on multiple nanopore sequencing devices. The user can select instructions specific to their device by selecting from the dropdown menu on the right hand side of the protocol.
August 14th, 2019RPB_9059_v1_revI_14Aug2019EnglishMinion Download 14th August 2019 The protocol has been expanded to include instructions for library prep on multiple nanopore sequencing devices. The user can select instructions specific to their device by selecting from the dropdown menu on the right hand side of the protocol.
August 2nd, 2019RPB_9059_v1_revH_08Mar2018English Download 19th July 2019 Protocol structure has been condensed to remove sections about DNA/RNA QC, software installation and use, and flow cell check. These sections are available as stand-alone documents, which are linked out from the protocol. The checklist protocol is now available for download from the button in the protocol header
June 28th, 2019RPB_9059_v1_revG_08Mar2018English Download Updated with new information for MinKNOW release v19.06: - Compression of queued reads - Pause functionality - UI improvements including: - Flow cell health at platform QC - Flow cell status on landing page - R10 flow cell running scripts - New Fast Models for RNA and DNA - PDF report and CSV files for Duty time and Cumulative output are now found in same location as actual reads and fastQ files - Flongle hardware check for confirming Flongle adapter functions correctly
June 12th, 2019RPB_9059_v1_revF_08Mar2018English Download Update to the flow cell priming section, and instructions for topping up the flow cell with fuel, following the release of the Flow Cell Priming Kit (EXP-FLP002)
May 2nd, 2019RPB_9059_v1_revE_08Mar2018English Download Updates for MinKNOW release 19.05
December 18th, 2018RPB_9059_v1_revD_08Mar2018English Download Updates for MinKNOW v18.12.4
September 3rd, 2018RPB_9059_v1_revC_08Mar2018English Download Updates for MinKNOW v2.2
July 19th, 2018RPB_9059_v1_revB_08Mar2018English Download Updates for MinKNOW GUI v2.0
March 22nd, 2018RPB_9059_v1_revA_08Mar2018English Download