Ligation sequencing amplicons - PCR barcoding (SQK-LSK109 with EXP-PBC001) (PBAC12_9067_v109_revV_14Aug2019)

DateVersionLanguageDeviceChanges
5/19/2023PBAC12_9067_v109_revV_14Aug2019EnglishGridION
May 2023 - Corrected the introduction for outlining the library prep
5/19/2023PBAC12_9067_v109_revV_14Aug2019EnglishMinION
May 2023 - Corrected the introduction for outlining the library prep
March 10th, 2023PBAC12_9067_v109_revU_14Aug2019EnglishGridion Download February 2023 - Updated product phase to 'Legacy'
March 10th, 2023PBAC12_9067_v109_revU_14Aug2019EnglishMinion Download February 2023 - Updated product phase to 'Legacy'
December 1st, 2022PBAC12_9067_v109_revT_14Aug2019EnglishGridion Download December 2022 - Updated concentration of first-round PCR to be consistent throughout the protocol
December 1st, 2022PBAC12_9067_v109_revT_14Aug2019EnglishMinion Download December 2022 - Updated concentration of first-round PCR to be consistent throughout the protocol
October 27th, 2022PBAC12_9067_v109_revS_14Aug2019EnglishGridion Download 27th October 2022 - Renamed "DNA repair and end-prep step" to "End-prep" and updated step to not use FFPE repair mix as DNA repair is not needed for amplicon input.
October 27th, 2022PBAC12_9067_v109_revS_14Aug2019EnglishMinion Download 27th October 2022 - Renamed "DNA repair and end-prep step" to "End-prep" and updated step to not use FFPE repair mix as DNA repair is not needed for amplicon input.
August 10th, 2022PBAC12_9067_v109_revR_14Aug2019EnglishGridion Download July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
August 10th, 2022PBAC12_9067_v109_revR_14Aug2019EnglishMinion Download July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
April 14th, 2022PBAC12_9067_v109_revQ_14Aug2019EnglishGridion Download 14th April 2022 Protocol name change
April 14th, 2022PBAC12_9067_v109_revQ_14Aug2019EnglishMinion Download 14th April 2022 Protocol name change
April 14th, 2022PBAC12_9067_v109_revQ_14Aug2019EnglishAny Download 14th April 2022 Protocol name change
October 15th, 2021PBAC12_9067_v109_revP_14Aug2019EnglishGridion Download October 2021 - Clarified flow cell loading recommendations for R10.3
October 15th, 2021PBAC12_9067_v109_revP_14Aug2019EnglishMinion Download October 2021 - Clarified flow cell loading recommendations for R10.3
September 13th, 2021PBAC12_9067_v109_revO_14Aug2019EnglishGridion Download 13th Sept 2021 - Added table outlining kit contents in the Equipment and consumables section
September 13th, 2021PBAC12_9067_v109_revO_14Aug2019EnglishMinion Download 13th Sept 2021 - Added table outlining kit contents in the Equipment and consumables section
January 20th, 2021PBAC12_9067_v109_revN_14Aug2019EnglishGridion Download 9th Dec Compatibilities updated for EXP-WSH004
January 20th, 2021PBAC12_9067_v109_revN_14Aug2019EnglishMinion Download 9th Dec Compatibilities updated for EXP-WSH004
February 21st, 2020PBAC12_9067_v109_revM_14Aug2019EnglishGridion Download 21st Feb 2020 Added 'Library storage recommendations' snippet before flow cell loading step
February 21st, 2020PBAC12_9067_v109_revM_14Aug2019EnglishMinion Download 21st Feb 2020 Added 'Library storage recommendations' snippet before flow cell loading step
October 23rd, 2019PBAC12_9067_v109_revL_14Aug2019EnglishGridion Download 27th September 2019 Updated and expanded Troubleshooting guide
October 23rd, 2019PBAC12_9067_v109_revL_14Aug2019EnglishMinion Download 27th September 2019 Updated and expanded Troubleshooting guide
August 23rd, 2019PBAC12_9067_v109_revK_14Aug2019EnglishGridion Download 23rd August 2019 Troubleshooting guide added to the protocol, detailing the most common issues seen during a nanopore sequencing experiment.
August 23rd, 2019PBAC12_9067_v109_revK_14Aug2019EnglishMinion Download 23rd August 2019 Troubleshooting guide added to the protocol, detailing the most common issues seen during a nanopore sequencing experiment.
August 14th, 2019PBAC12_9067_v109_revJ_14Aug2019EnglishGridion Download 14th August 2019 The protocol has been expanded to include instructions for library prep on multiple nanopore sequencing devices. The user can select instructions specific to their device by selecting from the dropdown menu on the right hand side of the protocol.
August 14th, 2019PBAC12_9067_v109_revJ_14Aug2019EnglishMinion Download 14th August 2019 The protocol has been expanded to include instructions for library prep on multiple nanopore sequencing devices. The user can select instructions specific to their device by selecting from the dropdown menu on the right hand side of the protocol.
July 19th, 2019PBAC12_9067_v109_revI_23May2018English- Download 19th July 2019 - Protocol structure has been condensed to remove sections about DNA/RNA QC, software installation and use, and flow cell check. These sections are available as stand-alone documents, which are linked out from the protocol. The checklist protocol is now available for download from the button in the protocol header - The cDNA option has been removed, and added to the Direct cDNA Sequencing Kit instructions instead.
June 28th, 2019PBAC12_9067_v109_revH_23May2018English- Download Updated with new information for MinKNOW release v19.06: - Compression of queued reads - Pause functionality - UI improvements including: - Flow cell health at platform QC - Flow cell status on landing page - R10 flow cell running scripts - New Fast Models for RNA and DNA - PDF report and CSV files for Duty time and Cumulative output are now found in same location as actual reads and fastQ files - Flongle hardware check for confirming Flongle adapter functions correctly
June 12th, 2019PBAC12_9067_v109_revG_23May2018English- Download Update to the flow cell priming section, and instructions for topping up the flow cell with fuel, following the release of the Flow Cell Priming Kit (EXP-FLP002)
May 2nd, 2019PBAC12_9067_v109_revF_23May2018English- Download Updates for MinKNOW release 19.05
April 30th, 2019PBAC12_9067_v109_revE_23May2018English- Download NEBNext® Companion Module for Oxford Nanopore Technologies® Ligation Sequencing now recommended for use together with the Ligation Sequencing Kit
December 18th, 2018PBAC12_9067_v109_revD_23May2018English- Download Updates for MinKNOW v18.12.4
October 15th, 2018PBAC12_9067_v109_revC_23May2018English- Download Updates to kit contents with the introduction of the Control Expansion kit (EXP-CTL001) and removal of the Lambda Control DNA from the Ligation Sequencing Kit
September 3rd, 2018PBAC12_9067_v109_revB_23May2018English- Download Updates for MinKNOW v2.2
August 9th, 2018PBAC12_9067_v109_revA_23May2018English- Download -