Ligation sequencing amplicons - PCR barcoding (SQK-LSK110 with EXP-PBC001) (PBAC12_9112_v110_revK_10Nov2020)

DateVersionLanguageDeviceChanges
3/10/2023PBAC12_9112_v110_revK_10Nov2020EnglishGridION
February 2023 - Updated product phase to 'Legacy'
3/10/2023PBAC12_9112_v110_revK_10Nov2020EnglishMinION
February 2023 - Updated product phase to 'Legacy'
December 1st, 2022PBAC12_9112_v110_revJ_10Nov2020EnglishGridion Download December 2022 - Updated concentration of first-round PCR to be consistent throughout the protocol
December 1st, 2022PBAC12_9112_v110_revJ_10Nov2020EnglishMinion Download December 2022 - Updated concentration of first-round PCR to be consistent throughout the protocol
October 27th, 2022PBAC12_9112_v110_revI_10Nov2020EnglishGridion Download 27th October 2022 - Renamed "DNA repair and end-prep step" to "End-prep" and updated step to not use FFPE repair mix as DNA repair is not needed for amplicon input.
October 27th, 2022PBAC12_9112_v110_revI_10Nov2020EnglishMinion Download 27th October 2022 - Renamed "DNA repair and end-prep step" to "End-prep" and updated step to not use FFPE repair mix as DNA repair is not needed for amplicon input.
August 10th, 2022PBAC12_9112_v110_revH_10Nov2020EnglishGridion Download 14th July 2022 - Updated compatibilities and removed references to discontinued flow cells
August 10th, 2022PBAC12_9112_v110_revH_10Nov2020EnglishMinion Download 14th July 2022 - Updated compatibilities and removed references to discontinued flow cells
July 4th, 2022PBAC12_9112_v110_revG_10Nov2020EnglishGridion Download 4th July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
July 4th, 2022PBAC12_9112_v110_revG_10Nov2020EnglishMinion Download 4th July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
April 13th, 2022PBAC12_9112_v110_revF_10Nov2020EnglishGridion Download 14th April 2022 Protocol name change
April 13th, 2022PBAC12_9112_v110_revF_10Nov2020EnglishMinion Download 14th April 2022 Protocol name change
February 24th, 2022PBAC12_9112_v110_revE_10Nov2020EnglishGridion Download 24th Febrary 2022 Updated EXP-AUX110 to new name EXP-AUX002
February 24th, 2022PBAC12_9112_v110_revE_10Nov2020EnglishMinion Download 24th Febrary 2022 Updated EXP-AUX110 to new name EXP-AUX002
January 26th, 2022PBAC12_9112_v110_revD_10Nov2020EnglishGridion Download 26th January 2022 - Updated mixing recommendations and adding tips regarding short term storage of the DNA library during the prep
January 26th, 2022PBAC12_9112_v110_revD_10Nov2020EnglishMinion Download 26th January 2022 - Updated mixing recommendations and adding tips regarding short term storage of the DNA library during the prep
September 13th, 2021PBAC12_9112_v110_revC_10Nov2020EnglishGridion Download 13th Sept 2021 - Added table outlining kit contents in the Equipment and Consumables section.
September 13th, 2021PBAC12_9112_v110_revC_10Nov2020EnglishMinion Download 13th Sept 2021 - Added table outlining kit contents in the Equipment and Consumables section.
January 20th, 2021PBAC12_9112_v110_revB_10Nov2020EnglishGridion Download 9th Dec Compatibilities updated for EXP-WSH004
January 20th, 2021PBAC12_9112_v110_revB_10Nov2020EnglishMinion Download 9th Dec Compatibilities updated for EXP-WSH004
November 10th, 2020PBAC12_9112_v110_revA_10Nov2020EnglishGridion Download -
November 10th, 2020PBAC12_9112_v110_revA_10Nov2020EnglishMinion Download -
October 20th, 2020PCB_amplicons_SQK-LSK110_v1EnglishGridion Download -
October 20th, 2020PCB_amplicons_SQK-LSK110_v1EnglishMinion Download -
October 20th, 2020PCB_amplicons_SQK-LSK110_v1EnglishAny Download -