Ligation sequencing gDNA - native barcoding (SQK-LSK109 with EXP-NBD196) (NBE_9121_v109_revK_19Jan2021)

DateVersionLanguageDeviceChanges
3/10/2023NBE_9121_v109_revK_19Jan2021EnglishGridION
February 2023 - Updated product phase to 'Legacy'
3/10/2023NBE_9121_v109_revK_19Jan2021EnglishMinION
February 2023 - Updated product phase to 'Legacy'
October 18th, 2022NBE_9121_v109_revJ_19Jan2021EnglishGridion Download 18th October 2022 - Updated equipment and consumables to remove reference to the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing
October 18th, 2022NBE_9121_v109_revJ_19Jan2021EnglishMinion Download 18th October 2022 - Updated equipment and consumables to remove reference to the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing
August 10th, 2022NBE_9121_v109_revI_19Jan2021EnglishGridion Download 14th July 2022 - Updated the compatibilities of the protocol and removed references to discontinued flow cells
August 10th, 2022NBE_9121_v109_revI_19Jan2021EnglishMinion Download 14th July 2022 - Updated the compatibilities of the protocol and removed references to discontinued flow cells
July 7th, 2022NBE_9121_v109_revH_19Jan2021EnglishGridion Download July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
July 7th, 2022NBE_9121_v109_revH_19Jan2021EnglishMinion Download July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
April 13th, 2022NBE_9121_v109_revG_19Jan2021EnglishGridion Download 14th April 2022 Protocol name change
April 13th, 2022NBE_9121_v109_revG_19Jan2021EnglishMinion Download 14th April 2022 Protocol name change
January 27th, 2022NBE_9121_v109_revF_19Jan2021EnglishGridion Download 27th January 2022 - Updated mixing recommendations and adding tips regarding short term storage of the DNA library during the prep In "Priming and loading the flow cell", further tips/notes have been added regarding preparing the library and flow cell loading.
January 27th, 2022NBE_9121_v109_revF_19Jan2021EnglishMinion Download 27th January 2022 - Updated mixing recommendations and adding tips regarding short term storage of the DNA library during the prep In "Priming and loading the flow cell", further tips/notes have been added regarding preparing the library and flow cell loading.
October 11th, 2021NBE_9121_v109_revE_19Jan2021EnglishGridion Download 11th Oct 2021 - Clarified the flow cell loading recommendations
October 11th, 2021NBE_9121_v109_revE_19Jan2021EnglishMinion Download 11th Oct 2021 - Clarified the flow cell loading recommendations
September 14th, 2021NBE_9121_v109_revD_19Jan2021EnglishGridion Download 14th Sept 2021 - In "Overview of the protocol" and "Equipment and consumables", there is a note that the 96 barcode orientation on the plate differs depending on the kit batch number
September 14th, 2021NBE_9121_v109_revD_19Jan2021EnglishMinion Download 14th Sept 2021 - In "Overview of the protocol" and "Equipment and consumables", there is a note that the 96 barcode orientation on the plate differs depending on the kit batch number
September 13th, 2021NBE_9121_v109_revC_19Jan2021EnglishGridion Download 13th Sept 2021 - Added tables outlining kit contents in teh Equipment and Consumables sections
September 13th, 2021NBE_9121_v109_revC_19Jan2021EnglishMinion Download 13th Sept 2021 - Added tables outlining kit contents in teh Equipment and Consumables sections
April 21st, 2021NBE_9121_v109_revB_19Jan2021EnglishGridion Download 21st April 2021 Inclusion of the optional Adapter Mix II Expansion (EXP-AMII001) kit for instances when a user is running a subset of barcodes in one experiment and needs extra Adapter Mix II for more reactions: - EXP-AMII001 is included in the "Equipment and consumables" section - Explanation of when to use EXP-AMII001 in the "Adapter ligation and clean-up" step
April 21st, 2021NBE_9121_v109_revB_19Jan2021EnglishMinion Download 21st April 2021 Inclusion of the optional Adapter Mix II Expansion (EXP-AMII001) kit for instances when a user is running a subset of barcodes in one experiment and needs extra Adapter Mix II for more reactions: - EXP-AMII001 is included in the "Equipment and consumables" section - Explanation of when to use EXP-AMII001 in the "Adapter ligation and clean-up" step
April 6th, 2021NBE_9121_v109_revA_19Jan2021EnglishGridion Download -
April 6th, 2021NBE_9121_v109_revA_19Jan2021EnglishMinion Download -
January 11th, 2021NBD_v109_revB_02July2020EnglishGridion Download 21st Feb 2020 Added 'Library storage recommendations' snippet before flow cell loading step
January 11th, 2021NBD_v109_revB_02July2020EnglishMinion Download 21st Feb 2020 Added 'Library storage recommendations' snippet before flow cell loading step
January 11th, 2021NBD_v109_revB_02July2020EnglishAny Download 21st Feb 2020 Added 'Library storage recommendations' snippet before flow cell loading step
July 2nd, 2020NBD_v109_revBEnglish- Download 21st Feb 2020 Added 'Library storage recommendations' snippet before flow cell loading step
July 2nd, 2020native_barcoding_96_v2EnglishAny Download 21st Feb 2020 Added 'Library storage recommendations' snippet before flow cell loading step
July 2nd, 2020native_barcoding_96_v2EnglishPromethion Download 21st Feb 2020 Added 'Library storage recommendations' snippet before flow cell loading step
July 2nd, 2020native_barcoding_96_v2EnglishGridion Download 21st Feb 2020 Added 'Library storage recommendations' snippet before flow cell loading step
July 2nd, 2020native_barcoding_96_v2EnglishMinion Download 21st Feb 2020 Added 'Library storage recommendations' snippet before flow cell loading step