RNA Leaders 2025

RNA Leaders is a must-attend event focused on RNA medicines such as RNAi, antisense, non-linear RNAs, RNA editing and targeting RNA, as well as manufacturing development of mRNA therapies and vaccine. The event features 4 simultaneous tracks covering in-depth development strategies, trends and technologies across the entire long and small RNA spectrum and areas of focus.

RNA Leaders Europe Tracks & Highlights:

1. Analytics & Discovery 2.Delivery
2. Next-Gen RNA
3. CMC & Scaling
4. Delivery & Process Development
5. Oligo-based Therapeutics
6. mRNA therapeutics & vaccines clinical advancements
7. Workshops and forums

Oxford Nanopore will be located at booth 7, and will host a seminar on Wednesday 5 March 2025.

Date: Wednesday 5 March, 2025

Time: 12.05 pm - 12:30 pm CET

Location: Delivery & Discovery Stream, RNA Leaders Europe

Title: Plasmid DNA and Direct RNA Sequencing for mRNA QC​

Description:

• Current mRNA characterization uses testing methods which require large capital investment, a considerable workforce, can be slow and cumbersome and do not always analyse the native molecule ​

• Explore rapid comprehensive Oxford Nanopore Technologies based CQA testing pipeline extending from the plasmid identity to the drug substance (mRNA) and drug product (mRNA) can complement state-of-the-art techniques by providing an orthogonal analysis while addressing current bottlenecks​

• Direct RNA chemistry enables sequencing RNA transcripts without the requirement of first converting to DNA, nor the need for PCR amplification ​

• Specific basecallers for transcripts containing RNA modifications, including N1-methyl-pseudouridine, which is relevant to mRNA production, and poly(A) tail length estimation​