Three High G+C soil proteobacterial genomes assembled using a hybrid approach

Three high G+C bacterial genomes were sequenced and assembled using a hybrid approach. Long scaffolding reads were obtained for all three organisms using the Oxford Nanopore MinION system, and short reads obtained through Illumina sequencing were used to alleviate non-random mutation in the final assemblies. The three soil bacteria sequenced were Variovorax paradoxus strain CSUSB, V. paradoxus strain VAI-C (a quorum sensing signal degrader), and a soil isolate of Pseudomonas alkylphenolica that was identified as tolerant to high levels of imidacloprid.

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