Nanopore direct RNA sequencing of human transcriptomes reveals the complexity of mRNA modifications and crosstalk between regulatory features
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- Published on: May 12 2025
- Source: Cell Genomics
Understanding the interactions between various mRNA features remains largely elusive due to the limitations of short-read sequencing technology. Kim and Saville et al. used Oxford Nanopore direct RNA sequencing to simultaneously analyse native mRNA modifications, splicing, and poly-A tail length in leukaemia cells, revealing their complex interactions. This high-resolution view offers new insights into transcript regulation and highlights the therapeutic relevance of targeting RNA modifiers like METTL3 in cancer.
Sample type: human cancer cell lines
