Targeted nanopore sequencing ushers in the era of routine long-read sequencing in translational research laboratories

Abstract

Nanopore sequencing offers attractive prospects for clinical laboratories with translational research activities: methylation analysis, variant phasing, gene fusion detection, and single nucleotide and structural variant detection. Using a collection of tumour and germline samples, we show how targeted sequencing with adaptive sampling enables the in-depth characterisation of relevant genomic regions on a single MinION Flow Cell per sample. This illustrates how nanopore sequencing can be made accessible for small-scale projects, and scalable to the systematic characterisation of samples in a translational research setting.

To learn more about other applications of nanopore sequencing in cancer research, click here.