Improving high-continuity plant de novo nanopore assemblies towards chromosome-scale reference genomes

Combining long-read nanopore sequencing with chromatin conformation capture or optical mapping enables the assembly of chromosome-level scaffolds for plant genomes.

Max and his team generated whole genome assemblies for lesser periwinkle, tomatilla, bladder cherry and tree tobacco.

‘Going full nanopore’: assembling tree tobacco with long nanopore reads and Pore-C chromatin conformation capture ‘worked really well for us’, producing a 3.6 Gb assembly with scaffold N50 59 Mbp.