Exploring the potential of chemo-enzymatic technology for improved detection of epigenetic modifications with nanopore sequencing
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We developed enzymatic technology that adds bulky chemical moieties to modifications, enhancing detection sensitivity during nanopore sequencing and allowing genome-wide detection with a small train-set. Specifically, we have shown that attachment of UDP-Glucose-Azide to 5hmC moieties significantly improves nanopore sensing ability, providing a promising avenue for characterizing other epigenetic modifications.